Vascular regression is crucial for limb development and pattern formation. We investigated this phenomenon using experimental modeling and mathematical approaches. In our in vitro model, we observed that Sox9 expression affects the behavior of endothelial cells, mimicking in vivo patterns. To further understand vascular regression, we developed a hybrid mathematical model that incorporates cell interactions, mechanics, and Sox9 pre-pattern formation. By combining experimental data and modeling, we gained insights into vasculature network formation and its role in organogenesis. Our interdisciplinary approach represents a first step in unraveling the vascular regression and advancing our understanding of limb development.